Abstract
BackgroundEmerged faba bean gall disease attacks the stem and leaves of the plant and results in complete crop losses. This study was initiated to screen multi-trait rhizobacteria for their antagonistic efficacy under in-vitro and in-vivo conditions against Olpidium viciae to control the effect of gall disease on faba bean. Sixty antagonistic isolates were first examined for their morphological, biochemical, and phenotypic traits.ResultsPseudomonas fluorescens AAUPF62, P. aeruginosa AAUS31, Bacillus AAUMF42, and Bacillus AAUAm28 showed greater than 68, 62, 57, and 54% suppression of O. viciae in dual culture, volatile metabolites, culture filtrate assay, and detached leaves experiments, respectively. The in-vivo study revealed that early treatment of the crop with P. aeruginosa AAUS31 reduced severity by 63% (in FB-Obse) and 54% (in FB-26869) faba bean varieties. The co-inoculation of P. fluorescens AAUPF62 and P. aeruginosa AAUS31 significantly enhanced the shoot (P = 0.003; mean = 122 cm) and root (P = 0.018; mean = 94 cm) length, increased shoot dry weight by 8 factors (83 g pot −1), and reduced final disease severity by 92% in FB-Obse variety.ConclusionsThe results revealed that P. fluorescens AAUPF62 and P. aeruginosa AAUS31 strains could be the potential antagonistic agents of gall disease. The use and early treatment of moderately resistant faba bean varieties by co-inoculation of synergistic potential bioagents were recommended.
Highlights
Emerged faba bean gall disease attacks the stem and leaves of the plant and results in complete crop losses
Among 60 potential antagonistic rhizobacteria obtained from rhizosphere of different faba bean were found belong to Pseudomonas (55%) and Bacillus (25%) genera
The most potential biological control rhizobacteria obtained were related to P. fluorescens (AAUPF62), P. aeruginosa (AAUS31), and the genera Bacillus (AAUMF42 and AAUAm28)
Summary
Emerged faba bean gall disease attacks the stem and leaves of the plant and results in complete crop losses. This study was initiated to screen multi-trait rhizobacteria for their antagonistic efficacy under in-vitro and in-vivo conditions against Olpidium viciae to control the effect of gall disease on faba bean. In recent years the newly emerged faba bean gall disease caused by plant debris and soil-borne fungal pathogen (Olpidium viciae Kusano) becomes one of the major faba bean production constraints in faba bean growing areas. More recently a good result has been obtained with beneficial bacteria that are indigenous to the rhizosphere of plants. Rhizobacteria are plant-associated bacteria, derived from many genera that can colonize roots and suppresses the growth of soil-borne phytopathogens through various mechanisms (Dinesh et al 2015). Pseudomonas and Bacillus species are among the rhizobacteria that can act against phytopathogens in plant root vicinity (Mota et al 2017)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
