Abstract
Background and Objective: Curative properties of Feijoa sellowiana Bert. (Myrtaceae, Acca sellowiana), have been often claimed, while investigations into details of its bioactivities are still lacking. This study investigated the effect of acetonic extract of Feijoa (Acca sellowiana (O. Berg) Burret) fruit on proliferation and apoptosis of bone marrow derived stem cells. Methodology: Human bone marrow stem cells (hBMSCs) were characterized before experiments. MTT Assay was performed to determine the toxicity of Feijoa. The growth kinetics was also investigated until 7 days. Real time PCR assessed the expression of apoptosis genes. Results: In MTT assay, Feijoa at doses ≤ 5 ng/ml did not show any cytotoxic effect on hBMSCs and increased the cell proliferation until day 4th followed by a decrease until day 7th. Population doubling time (PDT) decreased until day 4th followed by an increase until day 7th. A significant increase in expression of Bax and decrease Bcl-2 expression were recorded on day 7th. Conclusion: The antioxidant, anti-inflammatory and modulatory activities of Feijoa can explain the increasing effect on cell proliferation till day 4th, but the apoptotic activity of Feijoa noted after four days is reported for the first time denoting to the short term antioxidant, anti-inflammatory and modulatory properties of Feijoa that should be considered for curative activity of this fruit.
Highlights
In MTT assay, Feijoa at doses ≤ 5 ng/ml did not show any cytotoxic effect on Human bone marrow stem cells (hBMSCs) and increased the cell proliferation until day 4th followed by a decrease until day 7th
The antioxidant, anti-inflammatory and modulatory activities of Feijoa can explain the increasing effect on cell proliferation till day 4th, but the apoptotic activity of Feijoa noted after four days is reported for the first time denoting to the short term antioxidant, anti-inflammatory and modulatory properties of Feijoa that should be considered for curative activity of this fruit
When plotting the growth curve of hBMSCs exposed to 5 ng/mL of Feijoa fruit, an increasing trend in cell proliferation was noted till day 4th, followed by a decrease in cell proliferation after seven days, when compared to the control
Summary
Burret) known as Feijoa is a subtropical species of Myrtaceae family with medicinal properties that are cultivated in many countries including Iran [1,2,3] It has several varieties such as apollo, coolidge, gemini, mammoth, moore, and triumph with difference in size, maturation and quality of the fruit. It has essential minerals (e.g., potassium, calcium, magnesium, phosphorus, iodine), vitamins (e.g., vitamin C), terpenes, tannins, steroidal saponins, dietary fibers, flavonoids (e.g., catechin, flavone, quercetin-glycoside, procyanidin B1 and B2), phenolic acids, and polyphenols [6] These components are found in the pulp, and are identified in other parts of the plant such as leaf, peel and flower bud [4]. Berg) Burret) fruit on proliferation and apoptosis of bone marrow derived stem cells
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