Abstract
5 highly efficient hydrocarbon-degrading mixed bacteria were obtained from the petroleum-contaminated soil samples by the traditional method of enrichment and acclimation. The KL9-1 consortium had a wide temperature tolerance range and higher hydrocarbon degrading ability. The degradation rate of thin oil and heavy oil was up to 43.27% and 20.09% respectively through 7 d at 45oC. After several times of isolation and purification, three dominant strains with the capability of hydrocarbon-degrading were obtained, and their petroleum degradation rates were all over 30%. According to its morphological and 16S rDNA sequence characteristics, KL9-1-1 and KL9-1-2 were identified as Pseudomonas sp., and the KL9-1-3 was identified as Rhodococcus sp..
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