Screening for potential hemolytic responses to environmental agents using a bioactivation system: Evaluation of six pesticides

Abstract

Abstract Six pesticides were examined for oxidan stressor effects on Dorset sheep erythrocytes, an animal model with low glucose‐6‐ phosphate dehydrogenase (G‐6‐PD) activity. The pesticides tested were aldicarb, carbaryl, dluron, glyphosate, malathion and metolachlor. A liver microsomal enzyme system was used to generate metabolites of the parent compounds. The blood parameters examined for oxidant stressor effects were reduced glutathione (GSH) levels and methemoglobin (MetHb) formation. Acetylchol inesterase (ACHE) activity was measured after aldlcarb, carbaryl and metolachlor exposure. The results indicated that the parent compounds of all the pesticides and the metabolites of all pesticides except diuron were not oxldant stressor agents towards G‐6‐PD deficient erythrocytes, in vitro. Metabolites of dluron caused statistically significant increases In MEtHb levels of G‐6‐PD deficient erythrocytes. Significant reduction in erythrocyte ACHE levels were noted for metabolized and non‐metabolized aldicarb,...