Abstract
Polyhydroxyalkanoate (PHA) is a biodegradable material with many potential biomedical applications, including medical implants and drug delivery. This study developed a system for screening production strains in order to optimize PHA production in Cupriavidus taiwanensis 184, 185, 186, 187, 204, 208, 209 and Pseudomona oleovorans ATCC 29347. In this study, Sudan black B staining, Infrared (IR) and Gas Chromatography (GC) analysis indicated that the best strain for PHA synthesis is C. taiwanensis 184, which obtains polyhydroxybutyrate (PHB). Cultivation of C. taiwanensis 184 under a pH of 7.0, at 30 °C, and at an agitation rate of 200 rpm, obtained a PHB content of 10% and PHB production of 0.14 g/L. The carbon and nitrogen types selected for analysis of PHB production by C. taiwanensis 184 were gluconic acid and NH4Cl, respectively. Optimal carbon/nitrogen ratio for PHB production was also determined. This study demonstrated a PHB content of 58.81% and a PHB production of 2.44 g/L when the carbon/nitrogen ratio of 8/1 was selected for C. taiwanensis 184. A two-stage fermentation strategy significantly enhanced PHB content and PHB production. Under a two-stage fermentation strategy with nutrient-limited conditions, C. taiwanensis 184 obtained a PHB content of 72% and a PHB concentration of 7 g/L. Finally, experimental results confirmed that optimizing the growth medium and fermentation conditions for cultivating the indigenous C. taiwanensis 184 strain substantially elevated PHB content from 10% to 72% and PHB production from 0.14 g/L to 7 g/L, respectively.
Highlights
Rapid population growth in recent decades has resulted in severe environmental degradation.Because conventional plastics are not degradable by microorganisms, many companies have attempted to develop biodegradable alternatives
Polyhydroxyalkanoates (PHAs) are polyesters synthesized by various microorganisms, such as Ralstonia eutropha, Alcaligenes latus, Aeromonas hydrophila, Pseudomonas putida and Bacillus spp. [1,2,3,4,5]
The PHA production of the local indigenous strains was further evaluated by Gas Chromatography (GC)
Summary
Because conventional plastics are not degradable by microorganisms, many companies have attempted to develop biodegradable alternatives. Polyhydroxyalkanoates (PHAs) are polyesters synthesized by various microorganisms, such as Ralstonia eutropha, Alcaligenes latus, Aeromonas hydrophila, Pseudomonas putida and Bacillus spp. [1,2,3,4,5] Because of their good biodegradability and biocompatibility, PHAs have attracted interest in their use as an alternative to petroleum-based plastics including fine chemicals, plastics, printing materials, bio-fuel [6]. PHA properties for biomedical applications could be varied, based on the co-monomer structures of the copolymers [7]. The PHA types, such as polyhydroxybutyrate (PHB), poly(hydroxybutyrate-co-hydroxyvalerate)
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