Scopolamine and ketamine produce interaction on the contents of AchE and expression of NR2B mRNA in hippocampus of juvenile rats

Abstract

Objective To explore the interaction on the contents of AchE and expression of NR2B mRNA in hippocampus after combining scopolamine and ketamine to juvenile rats. Methods Forty one-month old qualified SD rats were divided at random into four groups with 10 rats each:control group(C group), scopolamine group (S group) , ketamine group(K group) , scopolamine and ketamine group(SK group). S,SK group were administered intraperitoneally scopolamine 0. 8 mg/kg;and after a half hour, K,SK group were administered intraperitone-ally ketamine 50 mg/kg, then KSK group were administered intraperitoneally ketamine 25 mg/kg every 30 minutes 2 times in total. C group were administered intraperitoneally equal volume physiological saline. The Y-maze was used to test the ability of learning on rats, then the animals were killed after the tests and left hippocampus were i-solated for determination of NR2B mRNA expression using RT-PCR technique. The right hippocampus were isolated for determination of AchE contents using colorimetry. Results SK group appeared much more difficult in learning, which training time and training quantity (45. 3 ±2. 9)min,(39.4 ±3.2) were respectively higher than S group(41.3 ±2.9)min,(35.0 ±4. 5) and K group(40. 5 ±2. 3)min, (36. 0 ±3.7). AchE contents and NR2B mRNA expression of SK group(2.46 ±0. 30,0. 72 ± 0. 05 ) were respectively higher than S group( 1. 98 ± 0. 25, 0.52 ± 0.04) and K group (1.91 ± 0. 28,0. 60 ± 0. 04) , there were significantly different (P < 0. 05 ). Conclusion After co-administration of scopolamine and kelamine in juvenile rats, it produces interaction on the contents of AchE and expression of NR2B mRNA in hippocampus, and inhibit learning more in rats. Key words: Cognition; Receptor; N-methyl-D-aspartate; Glutamic acid; Hippocampus