Abstract
Microporous and mesoporous zeolites, including ZSM-5, H-β, H-Y, and MCM-41, were modified with 3-aminopropyl-triethoxysilane (APTES), then inorganic fillers, such as abovementioned zeolites or mesoporous materials, (α-AlOOH or γ-Al2O3), were mixed with alginate embedded with yeast; and finally these carriers were cross-linked through the double oxirane. The alginate-based immobilized yeast with MCM-41 exhibited much shorter fermentation time and higher ethanol concentration than pure alginate and other composite carriers with the highest cell concentration of 4.8×109cells/mL. The composite carrier maintains the highest ethanol productivity of 6.55g/L/h for 60days in continuous fermentation process, implying good operational durability for commercial applications. The reason for the higher bio-catalytical function of the immobilized yeast might lay in the uniformly yeast distribution in the bio-reactor and high yeast cell concentration, which contributed by the improved transmission of fermentation media and combined effects of yeast adsorption by MCM-41 and embedment by alginate.
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