Abstract
Laboratory culture experiments were performed to optimize conditions for scaled-up production of Monostroma sp. The effects of growth determining factors like salinity (15–45 psu), photoperiod (8:16–16:8 L/D), light intensity (2–60 μmol photons m−2 s−1), and temperature (15–35 °C) on the growth of Monostroma sp. were assessed. Culture results indicated high tolerance capacity of Monostroma sp. to all levels in salinity, photoperiod, light intensity and temperature tested. ANOVA results confirmed significant impact of salinity, photoperiod, light intensity and temperature on growth rate (p < 0.05). The maximum growth rate was observed in the range of 5.73 to 14.41% day−1 which obtained in 35 psu salinity, 25 °C temperature, 14:10 (L/D) photoperiod, 60 μmol photons m−2 s−1 light intensity level and 1/4 MP1 medium. The modified 1/4MP1 medium was found to be suitable for enhanced growth of Monostroma sp. Outdoor tank culture with 1/4 MP1 medium showed maximum daily growth rate (14.38 ± 0.32% day−1).
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