Scalable Electro-Biosynthesis of Ectoine from Greenhouse Gases.

Abstract

Converting greenhouse gases into valuable products has become a promising approach for achieving a carbon-neutral economy and sustainable development. However, the conversion efficiency depends on the energy yield of the substrate. In this study, we developed an electro-biocatalyticsystem by integrating electrochemical and microbial processes to upcycle CO2 into a valuable product (ectoine) using renewable energy. This system initiates the electrocatalytic reduction of CO2 to methane, an energy-dense molecule, which then serves as an electrofuel to energize the growth of an engineered methanotrophic cell factory for ectoine biosynthesis. The scalability of this system was demonstrated using an array of ten 25 cm2 electrochemical cells equipped with a high-performance carbon-supported isolated copper catalyst. The system consistently generated methane at the cathode under a total partial current of approximately -37 A (~175 mmolCH4 h-1) and O2 at the anode under a total partial current of approximately 62 A (~583 mmolO2 h-1). This output met the requirements of a 3-L bioreactor, even at maximum CH4 and O2 consumption, resulting in the high-yield conversion of CO2 to ectoine (1146.9 mg L-1). This work underscores the potential of electrifying the biosynthesis of valuable products from CO2, providing a sustainable avenue for biomanufacturing and energy storage.