Abstract
Abstract An automated microarray diagnostic system for SARS-CoV-2 protein-specific antibodies was developed using photoimmobilized viral proteins. Photoimmobilization strategy was used to immobilize proteins by crosslinking reactions of photoreactive polymers. Aqueous solutions of proteins were spotted on a polymer-coated plate and dried in air. Then, the proteins were immobilized via irradiation with an ultraviolet lamp. Virus-specific IgG and IgM in the serum were automatically assayed using the microarray plate. After the serum was added on the microarray plate, peroxidase-conjugated anti-human-IgG or -IgM antibody was added, and the chemical luminescence intensity was detected using a sensitive charge-coupled device camera. The developed microarray assay showed higher sensitivity than the conventional immune chromatography. Additionally, compared with conventional microarray using nitrocellulose slide, the developed microarray format was useful for rapid assay formats in serodiagnosis of viral infectious diseases and assessment of immune status for clinical applications.
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