Abstract
Neutrophil extracellular traps (NETs) are DNA scaffolds released by activated neutrophils that contain enzymes from neutrophil granules, such as myeloperoxidase (MPO), neutrophil elastase (NE) and cathepsin-G. NETs are produced by neutrophils in response to various stimuli, including invasion of pathogenic microorganisms. Here we describe a new ELISA method for quantifying the circulating levels of MPO- and NE-associated DNA in human plasma.
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