Abstract
A rapid and sensitive sandwich electrochemical immunosensor was developed based on the fabrication of the graphene/polyaniline (GP/PANI) nanocomposite onto screen-printed gold electrode (SPGE) for detection of tuberculosis biomarker 10-kDa culture filtrate protein (CFP10). The prepared GP/PANI nanocomposite was characterized using Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FESEM). The chemical bonding and morphology of GP/PANI-modified SPGE were studied by Raman spectroscopy and FESEM coupled with energy dispersive X-ray spectroscopy, respectively. From both studies, it clearly showed that GP/PANI was successfully coated onto SPGE through drop cast technique. Cyclic voltammetry was used to study the electrochemical properties of the modified electrode. The effective surface area for GP/PANI-modified SPGE was enhanced about five times compared with bare SPGE. Differential pulse voltammetry was used to detect the CFP10 antigen. The GP/PANI-modified SPGE that was fortified with sandwich type immunosensor exhibited a wide linear range (20–100 ng/mL) with a low detection limit of 15 ng/mL. This proposed electrochemical immunosensor is sensitive, low sample volume, rapid and disposable, which is suitable for tuberculosis detection in real samples.
Highlights
One of the most critical infectious diseases is tuberculosis (TB), which can be fatal [1].Mycobacterium tuberculosis (M. tuberculosis) is the causative agent of TB which can be transmitted via minute aerosol droplets such as coughing, sneezing, or even talking by the infected TB person [2].This‘airborne contagious disease poses a particular infection-control challenge since the healthy personSensors 2018, 18, 3926; doi:10.3390/s18113926 www.mdpi.com/journal/sensorsSensors 2018, 18, 3926 can be infected by inhalation of an infectious droplet containing M. tuberculosis that suspended in the air
We presented a novel detection strategy that uses GP/PANI-modified screen-printed gold electrode (SPGE) and sandwich immunoassay of TB marker protein CFP10 for early detection of TB infection
This platform employs the capturewith antibody antibody (CapAb) immobilized with GP/PANI as an electrochemical transducer and the Ab was conjugated by Fe3 O4 /Au MNPs allowing for the antigen-specific detection of probes from the assay solution
Summary
Sensors 2018, 18, 3926 can be infected by inhalation of an infectious droplet containing M. tuberculosis that suspended in the air. One of the hallmarks of the M. tuberculosis is the ability of the bacteria to spread to other parts of the body through the blood circulation in the infected person. In this regard, the consequences of delayed diagnosis of TB are serious for both the prognosis of the patient and onward transmission to drive the epidemic. A diagnostic test that can be conducted at a single-visit, with theoretical 100% accuracy could save 625,000 lives a year if widely implemented [4]. Even a test with a sensitivity of only 85% and specificity of 97% could save 392,000 lives, equal to
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