Salt crystal deposition as a reversible mechanism to enhance photoprotection in black mangrove

Abstract

Mangrove forests are ecosystems made up of several woody plants living in saline coastal sedimentary habitats. In order to deal with the high salinity of the substrate, mangrove trees possess a number of different mechanisms to exclude, sequestrate or excrete the excess of salt. The black mangrove (Avicennia germinans L.), one of the dominant species in Central America, is characterized by high levels of salt excretion through epidermal glands. In this study, our aim was to examine whether, apart from its obvious role in salt tolerance, the formation of salt crystals on the upper leaf surface of black mangrove might represent an unusual and dynamic photoprotection mechanism. For this purpose, the reflection of light and a number of physiological parameters were studied during the dry and rainy seasons in black mangroves growing in the Juan Venado Island Nature Reserve (Nicaragua). Excreted salt increased the reflectance of the leaf surface mainly in the blue and red regions of the spectrum. By removing salt crust from the leaf surface, we demonstrated that during the most stressful periods (dry season at noon), this feature allowed leaves to maintain a higher photochemical efficiency and a lower leaf temperature as compared to uncovered leaves. Furthermore, this mechanism is fully reversible when conditions become more favorable, as salt crystals dissolve, forming drops. Thus, while being a detoxification mechanism developed mainly to avoid osmotic imbalance in the tissues, the excretion of salt through the leaves in black mangroves is an example of “exaptation”, as it has positive collateral effects on the photosynthetic performance of the plant, protecting A. germinans from overheating and photodamage during the harsher periods.