Salicylic acid alleviates the toxicity of cadmium on seedling growth, amylases and phosphatases activity in germinating barley seeds

Abstract

ABSTRACTThe present study investigated the possible mediator role of salicylic acid (SA) in alleviating cadmium (Cd) toxicity during the germination stage of barley. The exposure of barley seedling to increasing Cd concentrations (25, 50 and 100 µM) during early stages of their establishment, caused a gradual decrease in vigour index, root length, α-amylase, acid phosphatase, alkaline phosphatase activities in endosperms and mitotic index of the root tip. Seed pretreatment with 600 µM SA partially alleviated the negative effects of Cd on germination parameters and increased the hydrolytic enzyme activities and mitotic index. Cadmium treatment increased Cd accumulation in roots. Furthermore, results showed that compared to the cadmium treatment applied alone, SA pretreatment of the seeds did not influenced the Cd concentration in the roots. These results suggest that SA plays a positive role in barley-seed germination and early seedling growth by protecting it against Cd toxicity by moderating its toxic effect on the mobilization of organic reserves.