SAGE analysis of early oogenesis in the silkworm, Bombyx mori

Abstract

To identify genes involved in the differentiation of Bombyx cystoblast, we constructed two 3′ long serial analysis of gene expression (Long SAGE) libraries from stage 1–3 or stage 2–3 egg chambers and compared their gene expression profiles. In both libraries, the most frequent tags were derived from the same novel transcript. The transcript does not have any open reading frame capable of encoding a protein with over 100 amino acids in length. RNA blot analysis revealed that this transcript is specifically and abundantly expressed in the Bombyx ovary, mainly the germ line cells in the ovarioles. These results suggest that Bombyx oogenesis may be regulated by a previously unidentified non-coding RNA. Comparison of the gene expression profiles between the stage 1–3 and stage 2–3 egg chamber libraries revealed that 272 tags were significantly more abundant in stage 1–3 egg chambers ( p<0.05 and at least two-fold change) than in library 2. Among the differentially expressed transcripts were the sequences that correspond to ATP synthase subunit d (3.1-fold enriched) and ATP synthase coupling factor 6 (9.1-fold enriched), suggesting that they are involved in regulation of cell cycle of cystocytes.