Saccharum officinarum molasses adversely alters reproductive functions in male wistar rats

Abstract

The widely reported anti-androgenic effects of refined sugar led to the exploration of safer alternatives. Saccharum officinarum molasses (SOM), a byproduct of sugar processing is gaining popularity as a substitute. This study investigated the effects of SOM and compared them to those of refined sugar on male reproductive functions. Blackstrap® Saccharum officinarum molasses were subjected to phytochemical screening and proximate analysis and fractionated to obtain methanol (SOMMF) and aqueous (SOMAqF) fractions. Twelve groups (n = 5) of adult male Wistar rats received distilled water (Control); 0.8, 2.5, 7.9 g/kg SOM; 0.0064 g/kg sugar (Dangote®); 0.0064 g/kg sugar+7.9 g/kg SOM; 1.0, 3.2, 10.0 g/kg SOMMF and 0.6, 2.0, 6.4 g/kg SOMAqF, respectively. Administrations were done daily by oral gavage for eight weeks. Sperm profile and testicular and epididymal histology were assessed using microscopy. Serum testosterone was quantified using ELISA. Testicular malondialdehyde (MDA) was assayed by spectrophotometry. Data were analyzed using ANOVA at p < 0.05 significance. Sperm count and viability reduced with 7.9 g/kg SOM, Sugar, 3.2 and 10.0 g/kg SOMMF, 2.0 and 6.4 g/kg SOMAqF. Abnormal sperms increased with 7.9 g/kg SOM, Sugar, 2.0 and 6.4 g/kg SOMAqF. Testosterone level reduced with 6.4 g/kg SOMAqF. Testicular MDA increased with SOM, 3.2 and 10.0 g/kg SOMMF and 6.4 g/kg SOMAqF. Seminiferous tubules and epididymal ducts of 7.9 g/kg SOM, Sugar and SOMAqF-treated rats showed anomalies. Saccharum officinarum molasses altered testicular and epididymal integrity via lipid peroxidation, thus reducing sperm quality and androgen levels in male Wistar rats.