Abstract
This chapter presents a study examining S22 antibody as a new marker for mononuclear phagocytes in dermatopathology. Paraffin-embedded serial sections of sarcoidosis, mucocoele, and atopic eczema were selected because of the presumed presence of mononuclear phagocytes (MNPs) in the cellular infiltrate. The S22 antibody was used in a dilution of 1:400 in Tris buffered saline (TBS). Rabbit anti-human AT (Dakopatts) was used in a dilution of 1:800 in TBS. Rabbit anti-human ACT (Dakopatts) was used in a dilution of 1:200 in TBS. Rabbit anti-human LYZ (Dakopatts) was used in a dilution of 1:200 in TBS. Swine anti-rabbit antiserum (Dakopatts) was used as secondary antibody in a dilution of 1:100 in TBS. The rabbit PAP conjugate (Dakopatts) was used in a dilution of 1:400 in TBS. Positive cellular staining with all antisera was detected as a fine granular brown precipitate. At optimal dilutions, S22 was seen to label MNPs with an intensity equal to that seen with LYZ and stronger than that seen with alpha-1-antichymotrypsin (ACT) and alpha-1-antitrypsin (AT). There was minimal background staining with S22 and LYZ, but this was more marked with ACT and AT.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
