S215 – The Effects of Topical Halofuginon on Myringotomy in Rats

Abstract

Objectives 1) To investigate the role of halofuginone hydrobromide on myringotomy incision healing time. 2) To investigate the role of halofuginone hydrobromide on formation of myringosclerosis. Methods 40 rats with normal eardrums were involved in this study to determine if topical halofuginone application had an impact on prolonging the time for healing of myringotomy incision and its histopathological consequences. The study group and control group consisted of 30 and 10 rats, respectively. A posterior incision 1 mm in diameter was made on randomly selected healty eardrums of the rats. After making incision in the study group, gelfoam soaked in halofuginone hydrobromide of 30 mg/dl was applied on the perforation, while gelfoam dipped in isotonic saline was introduced to perforation in the control group. On days 1, 3,7,10,14,18,21, 24, 27 and 30, otoendoscopic evaluation of eardrums under general anesthesia was conducted and perforations were screened. On control days, one randomly selected rat from each group was sacrified and temporal bones were dissected in order to evaluate the tympanic membranes histopathologically. Results The average times for patency of perforation in study and control groups were 21.43 and 7.50 days, respectively. The difference was found to be statistically significant (p<0.05). Conclusions Histopathological evaluation revealed that halofuginone seems to result in low hyalinization and fibrosis on eardrum compared to the control group.