Abstract
l-[U- 14C]sucrose accumulation by phloem sieve tube members (PSTM) of wheat ( Triticum aestivum L. ‘Holley’) and sorghum ( Sorghum bicolor L. ‘G522 DR’) was inhibited by the nonpermeant sulfhydryl inhibitor p-chloromercuribenzenesulfonic acid (PCMBS), and this inhibition was reversed by the permeant sulfhydryl protectants dithiothreitol (DTT) and dithioerythritol (DTE). S-Ethyl dipropylthiocarbamate (EPTC) (≤0.1 m M) did not inhibit [ 14C]sucrose accumulation by wheat or sorghum PSTM. N-N-Diallyl-2-chloroacetamide (CDAA) (1 m M) inhibited [ 14C]sucrose accumulation by sorghum but not by wheat PSTM. The inhibition of [ 14C]sucrose accumulation in sorghum PSTM by the membrane permeant CDAA was reversed by DTT. Sorghum growth was inhibited by <1 μ M CDAA. Membrane permeant 2-chloroallyl diethyldithiocarbamate (CDEC) (0.1 m M) inhibited [ 14C]sucrose accumulation by PSTM of sorghum but not wheat. The inhibition of sucrose accumulation in sorghum PSTM by 0.1 m M CDEC was reversed by DDT.
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