Abstract
目的 探讨脊髓谷氨酸转运体(GT)、孤啡肽(OFQ)和脑源性神经营养因子(BDNF)在炎性痛大鼠吗啡耐受形成中的作用.方法 健康雄性SD大鼠,8～ 10周龄,体重300 ～ 350 g,取鞘内置管成功的雄性SD大鼠20只,采用随机数字表法,将其随机分为4组(n=5):生理盐水组(NS组)、炎性痛组(IP组)、吗啡组(M组)和GT激动剂riluzole组(R组).NS组于左后足踝关节腔注射生理盐水50μl,其余3组注射完全弗氏佐剂50 μl.3d后,NS组和IP组鞘内注射生理盐水10 μl;M组鞘内注射吗啡10 μg;R组鞘内注射riluzole 10 μg,30 min后注射吗啡10 μg.注射药物容量均为10μl,2次/d,连续7d.于鞘内给药前、鞘内给药2、4、6d和鞘内给药结束后1 d(T0-4)时测定机械痛阈.于T4时痛阈测定后取左侧脊髓背角,采用RT-PCR法测定OFQ mRNA和BDNF mRNA的表达.结果 与NS组比较,IP组机械痛阈降低,脊髓背角OFQ mRNA及BDNF mRNA表达上调(P＜0.05或0.01)；与IP组比较,M组T1,2时机械痛阈升高,脊髓背角OFQ mRNA及BDNF mRNA表达上调(P＜0.05或0.01),T3,4时机械痛阈差异无统计学意义(P＞0.05)；与M组比较,R组机械痛阈升高,脊髓背角OFQ mRNA及BDNF mRNA表达下调(P＜0.05或0.01).结论 炎性痛大鼠长期注射吗啡时脊髓GT功能降低,导致谷氨酸水平升高和OFQ、BDNF表达上调之间的失衡,可能在吗啡耐受形成中起到一定作用。
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
