Role of monocyte l-selectin in the development of post-traumatic organ failure

Abstract

The vascular leucocyte adhesion molecule, l-selectin, plays an important early role in monocyte trafficking at sites of inflammation, a process which leads to the development of inflammatory organ failure. In this prospective observational study, we investigate whether early numerical and functional changes in circulating monocytes, expression of monocyte l-selectin (CD62L) and monocyte:neutrophil l-selectin ratios are related to the subsequent development of post-traumatic organ failure (OF) and multiple organ dysfunction syndrome (MODS). Monocyte counts and cell surface l-selectin were measured by an automated cell counter and flow cytometry, respectively. Of 164 trauma patients admitted to a university emergency department resuscitation room, 64 had multiple injuries, 51 developed OF, 20 developed MODS and 21 died. Early monocyte counts in patients with multiple injuries were lower in those who developed MODS (0.44×10 9/l) compared with those who did not (0.60×10 9/l; P=0.024). Monocyte l-selectin mean channel fluorescence increased with injury severity and was highest in those who developed MODS ( P=0.033). In the sub-group of patients with multiple injuries, l-selectin mean channel fluorescence was also greater in those patients who developed MODS compared with patients who did not develop MODS ( P=0.042). The monocyte to neutrophil count ratio also decreased with injury severity ( P=0.006). Using optimal cut off values for l-selectin mean channel, fluorescence, the positive and negative predictive values for OF was 43.5 and 91.4%, respectively and for MODS it was 25.4 and 92.9%, respectively. Alterations in early circulating monocyte counts and l-selectin expression after injury are related to the development of post-traumatic organ failure and suggest an area in the inflammatory pathway that may be influenced by l-selectin blockade.