Abstract
Objective: The expression of fibroblast growth factor receptor 3 (FGFR3) in high-risk human papilloma virus (HPV)-infected tissues and its effect on the proliferation and differentiation of high-risk HPV-infected cells were studied. Methods: Immunohistochemistry was performed to determine FGFR3 expression in HPV-positive cutaneous squamous cell carcinoma (cSCC) tissues. Next, FGFR3 was knocked down in human keratinocytes, Ect1/E6E7 cells, and SiHa cells. An MTT assay was performed to determine cell proliferation, western blotting was used to detect the changes in phosphorylated FGFR3, and real-time quantitative polymerase chain reaction was used to detect the differentiation marker involucrin (INV). Results: The expression of FGFR3 in HPV-positive cSCC was higher than that in normal skin. After FGFR3 knockdown, the expression of FGFR3 and INV in SiHa cells significantly decreased (P < 0.001), the expression level of FGFR3 and INV mRNA also significantly decreased (P < 0.05), and changes in cell morphology and proliferation ability decreased (P < 0.05). Conclusion: FGFR3 is highly expressed in HPV-positive cSCC tissues. Decreased FGFR3 expression can inhibit proliferation and differentiation of HPV-positive cells.
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