Role of endogenous inhibitors on digestive amylolytic and proteolytic activities of a predatory hemipteran, Andrallus spinidens Fabricius

Abstract

The role of endogenous inhibitors on digestive amylolytic and proteolytic activities of Andrallus spinidens Fabricius (Hemiptera: Pentatomidae) were studied by using extracted endogenous inhibitors from Pieris brassicae, Naranga aenescens, Chilo suppressalis, Ephestia kuehniella and Hyphantria cunea. The endogenous inhibitors from P. brassicae and N. aenescens had the most inhibitory effects on α-amylase of A. spinidens. General proteolytic activity in the mid-gut of A. spinidens was affected by incubation of the endogenous inhibitors from N. aenescens, H. cunea and P. brassicae, but no inhibitory effects were observed in case of C. suppressalis and E. kuehniella. Extracted inhibitors from C. suppressalis, E. kuehniella and N. aenescens significantly decreased trypsin- and chymotrypsin-like activities in the mid-gut of A. spinidens. All extracted inhibitors significantly decreased elastase-like activity of A. spinidens vs. control, except for C. suppressslis. Extracted inhibitors from all species significantly decreased cathepisns B and L activities but this was not significantly different in case of C. suppressalis. Endogenous inhibitors also affected amino- and carboxypeptidase activities in the mid-gut of A. spinidens. Digestive enzymes of A. spinidens are sensitive to endogenous inhibitors from some preys and this might be the reason that some species are more appropriate than others.