Abstract
The southern root-knot nematode, Meloidogyne incognita, is one of the most prevalent and damaging plant-parasitic nematodes in the world and causes serious damages to agricultural production. We cloned a mitochondrial ATP synthase b subunit gene fragment of M. incognita (MiASB) based on the nematode genomics prediction. By soaking in the MiASB dsRNA solution, the hatching of RNAi treated eggs was reduced by 60% compared to negative control and by 64% compared to untreated control. Mortality of RNAi treated second stage juvenile (J2) was 8.6 times higher than that of negative control and 26 times higher than the untreated control. Inoculating the RNAi treated egg masses and J2 to tomato seedlings showed the pathogencity was significantly reduced. For the RNAi treated egg masses, the amount of root galls on silence treated seedlings was reduced by 92% compared to that on the negative control seedlings, and reduced by 93% compared to that on untreated control seedlings. For the treated J2, the amount of root galls on silence treated seedlings was reduced by 83% and 86% compared to negative and untreated control seedlings, respectively. The study revealed the MiASB silence had a positive effect on prevention and control of root-knot nematode disease, and also showed that the MiASB may be involved in the pathogenesis of nematode, which provided new ideas and ways to the research of nematode pathology and nematode disease control.
Highlights
Root-knot nematodes (RKN), genus Meloidogyne, are root endoparasites that can develop on a wide range of plant species
Using RNA interference (RNAi) by soaking it in the double-stranded RNA (dsRNA) solution of MiASB, we investigated the effects of MiASB on the hatching of the egg masses and the mortality of Meloidogyne nematode juveniles, and studied its inhibitory effect on nematode disease caused by M. incognita
A 650 bp fragment of MiASB was obtained by Polymerase chain reaction (PCR) using total RNA of M. incognita J2 as template
Summary
Root-knot nematodes (RKN), genus Meloidogyne, are root endoparasites that can develop on a wide range of plant species. The infective second stage juvenile (J2) penetrates the root tip and migrates intercellularly until it reaches the differentiating vascular cylinder [1]. It induces root-knot, stunts nutrient deficiency and disrupts the physiology of the host plants through their reproduction and feeding within plant roots, leading to a signifycant reduction in crop yield and deterioration of product quality [2,3]. The RKN is being controlled in the fields mainly based on cultural practices, host-plant resistance and the application of synthetic pesticides. Strategies based on the specific blocking of parasitism gene products involving in the success of infection would offer attractive alternatives to reduce nematode populations in the field [8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
