Abstract
Background Increasing cell specific productivity is still an important challenge in today’s production of biopharmaceuticals in mammalian cell cultures. During the last two decades various efforts were made to achieve higher yields, e.g. by optimization of process strategies, media development or cell line and metabolic engineering. Nevertheless, some challenges remain for efficient and applicable high titer production processes at industrial scale: the stability of engineered cell lines and the complexity of stable high-titer production processes as well as the characterization for regulatory authorities and strategies for downstream processing [1-3]. We found decreased transcript levels of the de novo methyltransferase 3a (Dnmt3a) in independent cultivation processes with increased productivity, i.e. in perfusions under glucose limitation and batch cultivation upon butyrate treatment. To prove the hypothesis that reduced transcript levels of Dnmt3a is responsible for an increase in productivity, we investigated the influence of RNAi-mediated knockdown of Dnmt3a in recombinant CHO cells on cell growth, viability and cellular and process productivity.
Highlights
Increasing cell specific productivity is still an important challenge in today’s production of biopharmaceuticals in mammalian cell cultures
To prove the hypothesis that reduced transcript levels of de novo methyltransferase 3a (Dnmt3a) is responsible for an increase in productivity, we investigated the influence of RNAi-mediated knockdown of Dnmt3a in recombinant CHO cells on cell growth, viability and cellular and process productivity
Upon butyrate treatment cell specific productivity increased in different CHO cell lines and process modi with decreased Dnmt3a expression levels
Summary
Increasing cell specific productivity is still an important challenge in today’s production of biopharmaceuticals in mammalian cell cultures. During the last two decades various efforts were made to achieve higher yields, e.g. by optimization of process strategies, media development or cell line and metabolic engineering. Some challenges remain for efficient and applicable high titer production processes at industrial scale: the stability of engineered cell lines and the complexity of stable high-titer production processes as well as the characterization for regulatory authorities and strategies for downstream processing [1-3]. To prove the hypothesis that reduced transcript levels of Dnmt3a is responsible for an increase in productivity, we investigated the influence of RNAi-mediated knockdown of Dnmt3a in recombinant CHO cells on cell growth, viability and cellular and process productivity
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
