RNA removal from Candida utilis by chemical and thermal treatments

Abstract

AbstractThe removal of the content of nucleic acids of fodder yeast (Candida utilis) by treatment with HCl or heat shock was investigatedAcid concentrations between 5 and 15% (on dried matter basis) were used. A maximal removal of the content of nucleic acids of 88% was realized wheńn was worked with 15% HCl, 90 °C during 30 minutes. But under this conditions were observed high demanges of protein and also of some essential amino acidsGood results for diminishing the content of nucleic acids were reached with the highest concentration of acid and a treatment during 20 minutesThe experiments with heat shock were carried out at 68 °C, different times for heating and different contents of yeastIn this way better results than for treatment with acid according to diminishing the content of nucleic acids and yield of protein and essential amino acids were reachedA removal of over 80% of the content of nucleic acids was achieved in all cases.