Review of the main traditional cheese-making in the Mediterranean basin

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Abstract The article delves into the rich history of dairy products, tracing their evolution from simple milk to a diverse array of derivatives, with specific focus on traditional cheese varieties originating from the Mediterranean region. It underscores the nutritional importance of dairy, emphasizing its abundance in vital nutrients such as proteins, lipids, and minerals. Our particular interest is the intricate process by which milk is transformed into cheese, a method that effectively preserves nutrients over extended periods. Enzymes play a key role in enhancing food quality throughout the production process. Despite the commercialization of certain cheese varieties, many remain unbranded, crafted for local consumption or distributed in an artisanal fashion. Nonetheless, the traditional quality associated with farm-based production is gradually waning in rural landscapes. The primary objective of the study is to meticulously identify and safeguard the legacy of traditional Mediterranean cheese varieties, recognizing their profound cultural and gastronomic significance. This research aims to preserve their vitality and support the conservation of an ancestral culinary heritage at risk of disappearing.

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Simple SummaryThe consumption of cheese is increasing from year to year, as this food is considered rich in nutrients, calcium and vitamins. Recently, cheese consumption has been associated with a lower incidence of cardiovascular diseases and type-2 diabetes. In this work, we characterized three traditional Egyptian cheeses (Karish, Domiati and Ras) and three additional cheese varieties consumed worldwide (Feta-type, Gouda and Edam) for their content of bioactive peptides (the main bioactive component of cheese responsible for its health benefits) and for their biological activities. Most of the identified bioactive peptides were able to inhibit key enzymes involved in the progression of cardiovascular diseases (angiotensin-converting enzyme, ACE) and diabetes (di-peptidyl-peptidase-IV, DPP-IV). Two well-known and studied bioactive peptides with anti-hypertensive activity in humans, called VPP and IPP, were quantified in the different cheeses. The results showed that the consumption of 10–20 g of Gouda cheese, 50–100 g of Domiati and 100 g of Edam should be enough to exert anti-hypertensive activity. Moreover, Gouda and Edam cheeses also contained a high amount of the DPP-IV-inhibitory peptide APFPE, suggesting a possible role in the prevention of diabetic complications.Several recent published studies reported that cheese consumption may protect against the onset of cardiovascular diseases and type-2 diabetes due to the presence of bioactive peptides. In the present work, six cheese varieties (the Egyptian traditional cheeses Karish, Domiati and Ras as well as Feta-type, Gouda and Edam cheeses) were characterized for their peptidomics profiles with high-resolution mass spectrometry, biological activities and content in bioactive peptides. The highest ACE-inhibitory and DPP-IV-inhibitory activities were found in Gouda cheese, which also displayed the highest antioxidant activity. A total of 809 peptides originating from the major milk proteins were identified, and 82 of them were bioactive. Most of them showed ACE-inhibitory, antioxidant and DPP-IV-inhibitory activities. The highest amount of the in vivo anti-hypertensive tripeptides VPP and IPP was found in Gouda cheese (39.19 ± 1.26 and 17.72 ± 0.89 mg/100 g of cheese, respectively), whereas the highest amount of APFPE was detected in Edam cheese (509.13 ± 20.44 mg/100 g of cheese). These results suggest that the intake of Edam, Domiati and, especially, Gouda cheeses may result in a possible anti-hypertensive effect in hypertensive subjects.

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The aim of the study was to determine the variability in the chemical composition and nutritive value parameters of smoked and unsmoked short-ripened rennet cheeses and unsmoked long-ripened rennet cheeses produced by traditional methods. The raw material for the production of short-ripened cheeses was pasteurized cows’ milk obtained from a dairy, whereas the long-ripened cheeses were manufactured from raw cows’ milk obtained from the producer’s farm. All three varieties of cheese examined were produced with commercial dairy starter cultures. The material for the study was collected in winter, directly at the producers’ retail outlets in southern and eastern Poland. The basic chemical composition was determined according to the Polish Standards, whereas the amino acid profiles of proteins from the cheeses were determined by ion-exchange chromatography. The result analysis revealed significant differences between the different varieties of cheese in terms of their water content, ranging from 32.2% to 42.1%, as well as protein content, which varied from 25.6% to 31.6%. Fat levels ranged between 22.2% and 24%, whereas total ash content amounted to 5.1-5.8%. The significantly highest salt content was found in unsmoked short-ripened cheeses. In all three cheese varieties, the total exogenous amino acid content was comparable, ranging from 46.17 g to 47.36 g/100 g protein, and that of endogenous amino acids varied from 52 g to 53 g/100 g protein. The biological value of proteins was determined by calculating to the chemical score (CS), as described by Mitchell and Block, and the essential amino acid index (EAAI), as described by Oser. A comparison of the results with the standard chicken egg white proteins showed that the limiting amino acids for all varieties of cheese were methionine and cysteine. On the other hand, a comparison with the FAO/WHO-suggested pattern of amino acid requirements (1991) for all age groups over 1 year of age showed that the limiting amino acids were methionine and cysteine in smoked and unsmoked short-ripened cheeses, and treonine in long-ripened cheeses. Considering the chemical indices, such as CS and EAAI, it may be concluded that the traditional rennet cheeses produced in southern and eastern Poland have a favourable amino acid composition of proteins and a high nutritive value..

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  • Cite Count Icon 5
  • 10.3390/plants12051154
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The herb of Trigonella caerulea (Fabaceae), commonly known as blue fenugreek, is used for the production of traditional cheese and bread varieties in the Alpine region. Despite its frequent consumption, only one study so far has focused on the constituent pattern of blue fenugreek, revealing qualitative information on some flavor-determining constituents. However, with regard to the volatile constituents present in the herb, the applied methods were insufficient and did not take relevant terpenoids into account. In the present study, we analyzed the phytochemical composition of T. caerulea herb applying a set of analytical methods, such as headspace-GC, GC-MS, LC-MS, and NMR spectroscopy. We thus determined the most dominant primary and specialized metabolites and assessed the fatty acid profile as well as the amounts of taste-relevant α-keto acids. In addition, eleven volatiles were quantified, of which tiglic aldehyde, phenylacetaldehyde, methyl benzoate, n-hexanal, and trans-menthone were identified as most significantly contributing to the aroma of blue fenugreek. Moreover, pinitol was found accumulated in the herb, whereas preparative works led to the isolation of six flavonol glycosides. Hence, our study shows a detailed analysis of the phytochemical profile of blue fenugreek and provides an explanation for its characteristic aroma and its health-beneficial effects.

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  • Cite Count Icon 13
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Proteomics Analysis in Dairy Products: Cheese, a Review
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  • Efterpi Bouroutzika + 5 more

Cheese is a worldwide produced and consumed commodity. There are many varieties of cheese from soft to hard, white to yellow, and fresh to aged after ripening. Especially, each category has its own producing technology. Many countries have labeled their most traditional cheese as Protective Designation of Origin (PDO). Moreover, several studies using advanced technologies, such as proteomics, have been performed to enhance labeling. In this review, broadly diffused and marketed, as well as Mediterranean countries’ special interest in Mediterranean diet-related PDO cheeses have been chosen as a reference. The aim of this work was to highlight the use of proteomics methods to examine how cheese proteins and peptides rearrange after ripening and use of starters. Further, we aimed to examine what kind of proteins are produced. Finally, we focused on bioactive molecules in cheeses and distinction of the original product from its counterfeit.

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