Abstract
Sheep brucellosis is a worldwide extended disease caused by B. melitensis and B. ovis, two species respectively carrying smooth or rough lipopolysaccharide. Vaccine B. melitensis Rev1 is used against B. melitensis and B. ovis but induces an anti-smooth-lipopolysaccharide response interfering with B. melitensis serodiagnosis, which precludes its use against B. ovis where B. melitensis is absent. In mice, Rev1 deleted in wbkC (Brucella lipopolysaccharide formyl-transferase) and carrying wbdR (E. coli acetyl-transferase) triggered antibodies that could be differentiated from those evoked by wild-type strains, was comparatively attenuated and protected against B. ovis, suggesting its potential as a B. ovis vaccine.
Highlights
Brucellosis is one of the most common zoonoses worldwide, causing significant loss to animal production and severely affecting human health [1]
We constructed a wbdR tagged Rev1 lacking N-formyl-perosamine in its O-PS (Rev1::Tn7wbdRΔwbkC) deleting wbkC from Rev1::Tn7wbdR. These constructs could be differentiated from Rev1, vaccine B. abortus S19, or representative strains of wild-type B. abortus, B. melitensis and B. ovis by PCR using the Multiplex-PCR combining Bruce-ladder and wbdR specific primers (Figure 1)
The results presented in this work demonstrate that the O-PS of vaccine Rev1 can be antigenically tagged by genomic insertion of wbdR, the acetyltransferase gene involved in the synthesis of the O-PS repeating unit of E. coli O157:H7
Summary
Brucellosis is one of the most common zoonoses worldwide, causing significant loss to animal production and severely affecting human health [1]. The etiological agents of brucellosis are Gram-negative bacteria of the genus Brucella. This genus includes several zoonotic species among which B. abortus preferentially infects cattle, B. suis swine and wild-life and B. melitensis goats and sheep [2]. Sheep can be infected by B. ovis, a nonzoonotic species [2]. These species are smooth (S) or rough (R), depending on the presence or absence respectively of O-polysaccharide (O-PS) chains in the outer membrane lipopolysaccharide (LPS). Whereas B. abortus, B. melitensis and B. suis carry a S-LPS, B. ovis is a naturally a R species [2]
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