Abstract
BackgroundThis study determines ‘correlation constants’ between the gold standard histological measurement of retinal thickness and the newer spectral-domain optical coherence tomography (SD-OCT) technology in adult C57BL/6 mice.MethodsForty-eight eyes from adult mice underwent SD-OCT imaging and then were histologically prepared for frozen sectioning with H&E staining. Retinal thickness was measured via 10x light microscopy. SD-OCT images and histological sections were standardized to three anatomical sites relative to the optic nerve head (ONH) location. The ratios between SD-OCT to histological thickness for total retinal thickness (TRT) and six sublayers were defined as ‘correlation constants’.ResultsMean (± SE) TRT for SD-OCT and histological sections was 210.95 µm (±1.09) and 219.58 µm (±2.67), respectively. The mean ‘correlation constant’ for TRT between the SD-OCT and histological sections was 0.96. The retinal thickness for all sublayers measured by SD-OCT vs. histology were also similar, the ‘correlation constant’ values ranged from 0.70 to 1.17. All SD-OCT and histological measurements demonstrated highly significant (p<0.01) strong positive correlations.ConclusionThis study establishes conversion factors for the translation of ex vivo data into in vivo information; thus enhancing the applicability of SD-OCT in translational research.
Highlights
Spectral-domain optical coherence tomography (SD-OCT) is an important imaging modality, in clinical ophthalmology and animal research, for characterizing morphology and understanding pathophysiological changes in the retina
High resolution detailed images comparable to mid- to high-range microscope objective lens is attained with newer generation OCT technology [4]
Ocular histology is still considered to be the gold standard for assessing structural morphology
Summary
Spectral-domain optical coherence tomography (SD-OCT) is an important imaging modality, in clinical ophthalmology and animal research, for characterizing morphology and understanding pathophysiological changes in the retina. The rapid, high resolution, non-invasive cross sectional images produced by SDOCT is an important tool in diagnosing posterior segment pathology and monitoring it longitudinally [1,2]. SDOCT provides non-invasive histological–grade sections of the rodent posterior segment, image acquisition is technologically cumbersome as human devices are retrofitted for animal use. High resolution detailed images comparable to mid- to high-range microscope objective lens is attained with newer generation OCT technology [4]. This study determines ‘correlation constants’ between the gold standard histological measurement of retinal thickness and the newer spectral-domain optical coherence tomography (SD-OCT) technology in adult C57BL/6 mice
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