Abstract
Background: Beneficial effects of Resveratrol (RSV) have been demonstrated, including effects on transporters and channels. However, little is known about how RSV influences intestinal transport. The aim of this study was to further characterize the effects of RSV on intestinal transport and the respective mechanisms. Methods: Porcine jejunum and ileum were incubated with RSV (300 µM, 30 min) in Ussing chambers (functional studies) and tissue bathes (detection of protein expression, phosphorylation, association with detergent resistant membranes (DRMs)). Results: RSV reduced alanine and glucose-induced short circuit currents (ΔIsc) and influenced forskolin-induced ΔIsc. The phosphorylation of sodium–glucose-linked transporter 1 (SGLT1), AMP-activated protein kinase (AMPK), protein kinase A substrates (PKA-S) and liver kinase B1 (LKB1) increased but a causative relation to the inhibitory effects could not directly be established. The DRM association of SGLT1, peptide transporter 1 (PEPT1) and (phosphorylated) Na+/H+-exchanger 3 (NHE3) did not change. Conclusion: RSV influences the intestinal transport of glucose, alanine and chloride and is likely to affect other transport processes. As the effects of protein kinase activation vary between the intestinal localizations, it would appear that increasing cyclic adenosine monophosphate (cAMP) levels are part of the mechanism. Nonetheless, the physiological responses depend on cell type-specific structures.
Highlights
Resveratrol (RSV) is supposed to mediate beneficial effects on a wide variety of physiological parameters
As the effects of protein kinase activation vary between the intestinal localizations, it would appear that increasing cyclic adenosine monophosphate levels are part of the mechanism
With regard to the objectives of this study, it can be summarized that Na+-dependent alanine transport is inhibited by RSV and that the segment-specific characteristics are similar to those observed transport is inhibited by RSV and +that the segment-specific characteristics are similar to those for the RSV-induced inhibition of Na -dependent glucose transport, while no alterations in the activity observed for the RSV-induced inhibition of Na+-dependent glucose transport, while no alterations in of Na+ /K+ -ATPase+ were observed
Summary
Resveratrol (RSV) is supposed to mediate beneficial effects on a wide variety of physiological parameters. In a previous study on the influence of RSV on intestinal glucose absorption, we showed in Ussing chamber studies that short-time incubation with RSV (30 min) decreases the electrogenic Na+ -dependent glucose transport (measured as change in short circuit currents (Isc )) in jejunal and ileal samples of porcine. Methods: Porcine jejunum and ileum were incubated with RSV (300 μM, 30 min) in Ussing chambers (functional studies) and tissue bathes (detection of protein expression, phosphorylation, association with detergent resistant membranes (DRMs)). ∆Isc. The phosphorylation of sodium–glucose-linked transporter 1 (SGLT1), AMP-activated protein kinase (AMPK), protein kinase A substrates (PKA-S) and liver kinase B1 (LKB1) increased but a causative relation to the inhibitory effects could not directly be established. The physiological responses depend on cell type-specific structures
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