Abstract
BackgroundResveratrol is reported to inhibit the growth of prostate, which is characteristic of benign prostatic hyperplasia (BPH) condition. However, the mechanism remains unclear. This study aimed to identify the effects and probable mechanism of resveratrol on BPH.MethodsWe used the BPH epithelial cell line BPH-1 to investigate the effect of resveratrol. Cells were treated with various concentrations of resveratrol, and its effects on cells viability, apoptosis, ROS accumulation, and cell cycle were assessed. Western blot was used to examine activation of p38 MAPK and protein levels of FOXO3a, Bcl2, Bcl-XL, and caspase3. Cells were also co-treated with the p38 MAPK inhibitor SB203580 or ROS scavenger N-Acetyl-L-cysteine (NAC) to further investigate the mechanism.ResultsResveratrol treatment inhibited the growth of BPH-1 and increased apoptosis of cells. In addition, levels of phosphorylated p38 MAPK level was elevated and FOXO3a repression was observed. Concomitantly, ROS was accumulated. All of these resveratrol-mediated effects were suppressed by additional treatment with SB203580 or NAC. Resveratrol was also found to induce cell cycle arrest at S phase.ConclusionsResveratrol can activate p38 MAPK and repress FOXO3a, thereby causing repression of SOD2, catalase, and increase of ROS accumulation, leading to apoptosis in BPH-1 cells.
Highlights
Resveratrol is reported to inhibit the growth of prostate, which is characteristic of benign prostatic hyperplasia (BPH) condition
Resveratrol inhibited the growth and promoted apoptosis of BPH-1 To investigate the effect of resveratrol on BPH, we used the BPH-1 cell line as our model
The BPH-1 cells were treated with various concentrations of resveratrol for two days, after which the cell viability was markedly reduced in a time and dose-dependent manner (Fig. 1e)
Summary
Resveratrol is reported to inhibit the growth of prostate, which is characteristic of benign prostatic hyperplasia (BPH) condition. This study aimed to identify the effects and probable mechanism of resveratrol on BPH. BPH patients experience frequent micturition or urgent urination, especially at night [3, 4]. The exact mechanisms and Therapy for BPH consists of medical treatment and surgery. Traditional therapy for BPH involves administration of 5α-reductase inhibitors and α blockers, which can shrink the prostate gland or alleviate bladder outlet obstruction (BOO). These treatments have many side effects, such as gynecomastia and sexual dysfunction, and is not effective in all patients [7]. There is a need for more efficient drugs with less side effects to treat BPH
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