Abstract
This study was carried out for detected of the restriction map of tcdB gene for A - B + isolates were isolated from patients with a colitis disease in General Basrah Hospital by (AluI, DraI, ScaI) restriction enzymes.The genetic restriction map was detected by determining the locations of a number of restriction endonuclease cleavage sites on the DNA relative to each other. AluI enzyme was showed three bands (100 bp, 90 bp and 60 bp) revealing there are two sites of this enzyme, on the other hand DraI, ScaI gave two bands (125 bp) for each enzyme revealing there was a single site of these enzymes, A variety of tcdB gene which detected in this study a new modification increasing the virulence of A - B + isolates of C. difficile with the large defect of tcdA gene.
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