Abstract
The regulatory factor X (RFX) family of transcription factors is characterized by a unique and highly conserved 76-amino acid residue DNA-binding domain. Mammals have five RFX genes, but the physiological functions of their products are unknown, with the exception of RFX5. Here a mouse RFX4 transcript was identified that encodes a peptide of 735 amino acids, including the DNA-binding domain. Its expression was localized in the suprachiasmatic nucleus, the central pacemaker site of the circadian clock. Also, light exposure was found to induce its gene expression in a subjective night-specific manner. Polyclonal antibodies were prepared, and an 80-kDa band was detected in the suprachiasmatic nucleus by Western hybridization. A histochemical study showed a localization of the products in the nucleus. This is the first report on mouse RFX4, which contains the RFX DNA-binding motif. Our investigation may provide clues to the physiological function of RFX4.
Highlights
Regulatory factor X (RFX)1 is a DNA-binding protein that recognizes the X-box sequence in the transcription regulation region of major histocompatibility complex class II genes [1]
A Novel Form of Mouse RFX4 Transcript Is Expressed in the suprachiasmatic nucleus (SCN)—Recently, the full-length human RFX4 cDNA was isolated, and the interaction of its product with other RFX products was demonstrated
The RFX4 (AK016791) transcript was identified in the testis, but it lacks the RFXspecific DNA-binding domain (DBD) [30]
Summary
Regulatory factor X (RFX)1 is a DNA-binding protein that recognizes the X-box sequence in the transcription regulation region of major histocompatibility complex class II genes [1]. A mouse RFX4 transcript was identified that encodes a peptide of 735 amino acids, including the DNA-binding domain. 1 The abbreviations used are: RFX, regulatory factor X; DBD, DNAbinding domain; DIM, dimerization; SCN, suprachiasmatic nucleus; LD, light/dark; DD, constant darkness; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; CT, circadian time; EST, expressed sequence tag; RT, reverse transcriptase.
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