Abstract
To examine activity of estrogen receptor-beta (ERβ) independently of estrogen receptor-alpha (ERα), retrovirus-mediated gene transfer was used to insert rat ERβ into a rat fibroblast cell line (rat-1) that does not ordinarily express ER. Stable expression of ERβ in rat-1 cells was validated and then characterized by reverse-transcription polymerase chain-reaction (RT-PCR) analysis to examine the effects of estradiol (E 2) treatment on expression of specific target mRNAs. Results were compared with rat-1 cells and a previously constructed rat-1+ERα cell line. Progesterone receptor mRNA was not detected in rat-1 cells and was induced by E 2 in both rat-1+ERα and rat-1+ERβ cells. Treatment with E 2 resulted in an increased rate of cell proliferation ( P<0.05) in rat-1+ERα cells, but not in rat-1 or rat-1+ERβ cells. Data confirm studies using transient ER expression demonstrating that ERα and ERβ have both discrete and overlapping activity within the same cell type in the presence of the same ligand.
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