Abstract
The effects of benzo( a)pyrene (BaP), benzo( k)fluoranthene (BkF) and their mixture on antioxidant enzyme activities and lipid peroxidation (LPO) levels of haemolymph of scallop ( Chlamys ferrari) were studied. The superoxide dismutase (SOD) activities of 0.5 μg/L and 1.0 μg/L were significantly higher than controls ( P < 0.05), while it increased at beginning and then dropped (lower than controls) in the end at 10.0 μg/L and 50.0 μg/L PAHs groups. The catalase (CAT) activities were very little during the whole experimental time. The glutathione peroxidase (GPx) activities in each PAHs group all increased significantly ( P < 0.05). LPO levels all increased significantly ( P < 0.05) with time at each PAHs group except for the 0.5 μg/L group of less than hour 12. The toxicity of PAHs in a descending order was BaP > BkF > mixture of BaP and BkF. The changes in antioxidant enzyme activities and LPO level in haemolymph could reflect the detoxification functions and damage levels of whole organism.
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