Abstract
Galla Chinensis was a traditional Chinese medicine used as an ingredient in many prescriptions for the treatment of oxidant damage-associated symptoms in China. The current research on the isolation of polysaccharides from Galla Chinensis and their antioxidant activities still remain as an ongoing challenge. The purpose of this study is to develop an isolation technique for polysaccharides from Galla Chinensis and determine their antioxidant properties in vitro. An ultrasonic-assisted method of polysaccharides extraction from Galla Chinensis were firstly developed and the key parameters were optimized response surface analysis as crushing degree 100 mesh, ultrasonic time 20 min, and materials/liquid ratio 1: 40. The polysaccharides presented dose-dependent antioxidant and free radical scavenging effects in DPPH, ABTS, total antioxidant and Iron ion reduction assays. Moreover, it also demonstrated an effective protective effect for human under H2O2-induced oxidative damage on human embryonic kidneyfibroblast 293 and hepatoma 7721 cells by cell proliferation assay. The study firstly determined the antioxidant activities of polysaccharides from Galla Chinensis, thus providing a new natural resource for future investigation and development of the polysaccharides-based antioxidant drugs, health products or addictives.
Highlights
Galla Chinensis, used as a Traditional Chinese Medicine (TCM), is in a form of the gall produced through parasitic interaction between plants (family Anacardiaceae, mainly Rhus chinensis Mill, Rhus potaninii Maxim, and Rhus punjabensis var. sinica (Diels) Rehd
In the single factor experiment, crushing degree, materials/liquid ratio, ultrasonic times and temperature were selected to be the parameter optimized during the polysaccharides isolation of Galla Chinensis
An ultrasonic-assisted method has been developed to extract the polysaccharides from Galla Chinensis through response surface optimization
Summary
Galla Chinensis, used as a Traditional Chinese Medicine (TCM), is in a form of the gall produced through parasitic interaction between plants (family Anacardiaceae, mainly Rhus chinensis Mill, Rhus potaninii Maxim, and Rhus punjabensis var. sinica (Diels) Rehd. EtWils) and insects (Chinese sumac aphid Baker, mainly Melaphis chinensis Bell) (State Pharmacopoeia Committee of the People’s Republic of China, 2015) This horned and reddish-brown gall, covered with the velvety down, is traditionally used to treat various body inflammatory diseases, such as toothache, dental caries, diarrhea, dysentery, painful hemorrhoids, and cancers in the many northeast and southeast Asian countries, especially in China (Sariozlu & Livanc, 2011; Wang et al, 2019). Ellagic acid, β-sitosterol, amentoflavone and purpurogallin were most frequently investigated and considered as the antioxidant compounds of Galla Chinensis Their protective role in preventing various oxidative damage diseases have widely reported toward the various application potentials in recent years (Chen et al, 2018; Farbood et al, 2019; Kahkeshani et al, 2019; Ponnulakshmi et al., 2019; Xie et al, 2019). The understanding of its phytochemical knowledge clearly needs further complement
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