Abstract
Stenotrophomonas maltophilia AAP56, laccase-producing bacteria, growing under different conditions, exhibit laccase activity that is highly affected by some environmental factors. Response surface methodology (RSM) was applied for the determination of laccase factor dependence using two substrates: ABTS (2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)) and DMP (2,6 Dimethoxy-phenol). RSM was carried out with a 24 factorial design using four variables, namely, dye (0 to 0.1 mg mL-1), Cu in Med (0 to 400 µM), shaking (0 to 150 rpm), and CuSO4 in assay (0 to 0.2 mM). Significant correlation between the effects of these variables on R1 (ABTS oxidase activity) and R2 (DMP oxidase activity) responses was detected. Astonishing results showed differences between these two activities with respect to copper activity dependence. Anoxic conditions exhibited a significant ability to induce the enzyme. This bacterial laccase activity (produced under optimal conditions according to RSM) was used to decolorize an azoic dye, Reactive Black 5 (RB5). It was efficient only in the presence of a redox mediator to degrade RB5 after 20 min of incubation time.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
