Abstract
Summary. A trial of factorial design is described, in which was studied the response of the cyclic ewe to a horse anterior pituitary extract (hap) Three total-dose levels of hap (60, 90 and 135 mg) were administered over 2 or 3 days commencing on the 12th day of the oestrous cycle. The total dose was given as either two or three equal daily injections, or four or six equal twice-daily injections. Highly significant (P<0\m=.\001) linear dose-response relationships were observed in the ovulatory response to both 2- and 3-day treatments. Treatment for 3 days was significantly better than treatment for 2 days in the proportion of ewes ovulating (P<0\m=.\05), ovulation rates (P<0\m=.\001) and fertilization of eggs shed (P< 0\m=.\001). For both 2- and 3-day treatments the ovarian responses varied with the time elapsing between the final hap injection and the onset of oestrus. Maximum responses were observed in ewes in oestrus 24 to 48 hr after the cessation of treatment. Fertilization in ewes treated for 2 days, but not in those treated for 3 days, also varied with the time elapsing between the cessation of treatment and onset of oestrus. The results showed hap to be a most effective preparation for the induction of multiple ovulation in the ewe.
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