Abstract
Bovine and porcine peripheral blood mononuclear cells (PBMC) were tested for their response to human recombinant interleukin 2 125 (rIL 2 125). The rIL 2 125 used in these experiments was purified to homogeneity from Escherichia coli , contained a site-specific modification at amino acid #125 replacing a cysteine with a serine residue and had a specific activity of 4×10 6 units/mg. Human rIL 2 125 was shown to be directly mitogenic for bovine and porcine PBMC and was able to maintain the long-term growth of mitogen-activated PBMC of both species. Long-term cultures were highly sensitive to low levels of rIL 2 125 and showed dose-dependent responses when used in short-term IL 2 assays. Bovine and porcine PBMC preincubated with human rIL 2 125 for 1 and 5 days demonstrated enhanced levels of cell-mediated cytotoxicity against both allogeneic and xenogeneic cell lines.
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