Abstract
Abstract. In order to test the influences of ocean acidification on the ocean pelagic ecosystem, so far the largest CO2 manipulation mesocosm study (European Project on Ocean Acidification, EPOCA) was performed in Kings Bay (Kongsfjorden), Spitsbergen. During a 30 day incubation, bacterial diversity was investigated using DNA fingerprinting and clone library analysis of bacterioplankton samples. Terminal restriction fragment length polymorphism (T-RFLP) analysis of the PCR amplicons of the 16S rRNA genes revealed that general bacterial diversity, taxonomic richness and community structure were influenced by the variation of productivity during the time of incubation, but not the degree of ocean acidification. A BIOENV analysis suggested a complex control of bacterial community structure by various biological and chemical environmental parameters. The maximum apparent diversity of bacterioplankton (i.e., the number of T-RFs) in high and low pCO2 treatments differed significantly. A negative relationship between the relative abundance of Bacteroidetes and pCO2 levels was observed for samples at the end of the experiment by the combination of T-RFLP and clone library analysis. Our study suggests that ocean acidification affects the development of bacterial assemblages and potentially impacts the ecological function of the bacterioplankton in the marine ecosystem.
Highlights
Hydrology and Microorganisms are a food webs (Azam et akle.,y1c9o8m3)pS.oEcneuinektanriyncometicasripnheytpolpanlakntkotnoinc and cyanobacteria contribute significantly to photosynthesis and primary production in the ocean
The e-digestion analysis of clone library sequences suggested that two Actinobacteria groups (Ilumatobacter and Cryobacterium) produced this T-RF
This was supported by the finding that the clone libraries were dominated by clones affiliated to these two Actinobacteria groups (Table 2)
Summary
Hydrology and Microorganisms are a food webs (Azam et akle.,y1c9o8m3)pS.oEcneuinektanriyncometicasripnheytpolpanlakntkotnoinc and cyanobacteria contribute significantly to photosynthesis and primary production in the ocean. Heterotrophic bacteria and archaea process approximate half of the primary production and recycle orOgacniecaannd Sinocrigeanniccenutrients These microbial functions are crucial components in several major concepts of marine food web structure and organic matter cycling, e.g., the microbial loop and microbial carbon pump (Azam et al, 1983; Jiao et al, 2010). Contribute to microbial biogeography in the ocean (Martiny edtuacle.,d2b0y06an).tChroonpsoigdeeTnriinchgiemthpeCacchrtasyn(oginisnclgpuodhcineegarnceilcimcoantedictihoannsgien)-, it is necessary to investigate whether and how the microbial communities would respond to the emerging changes, and to evaluate the possible ecological consequences of the microbial responses Such hitherto unknown information is crucial to our understanding and predictions of the possible effects of global climate changes to the biosphere (IPCC, 2007)
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