Abstract
TANOOKA, H. and TERANO, H. Resistance of DNA against RadiationInduced Strand Breakage in Bacterial Spores. Radiat. Res. 43, 613-626, (1970). Single- and double-strand breaks of DNA in electron-irradiated Bacillus subtilis spores were measured and compared with those in irradiated vegetative cells by zone sedimentation in alkaline and neutral pH sucrose gradients. DNA in spores was much more resistant to both types of damage than DNA in vegetative cells. Induction of one single-strand break in DNA of spore genome required 2.8 krad compared to 0.13 krad in vegetative cell genome. In other terms, energies of 857 eV in spore DNA and 41 eV in vegetative cell DNA were required to induce one single-strand break, calculated from molecular weights given by weight-average sedimentation distances; or 835 eV and 46 eV, calculated from weight-average molecular weights. The resistance of DNA was maintained after spores were converted to osmotically sensitive spheroplasts; however, the resistance was lost by rupture of the spheroplast structure. This resistance of DNA was not due to the repair activity of spores nor to protective substance in spore
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
