Abstract
The excellent survival ability of Salmonella enterica serovar Enteritidis (S. Enteritidis) in egg white leads to outbreaks of salmonellosis frequently associated with eggs and egg products. Our previous proteomic study showed that the expression of multidrug efflux RND transporter AcrD in S. Enteritidis was significantly up-regulated (4.06-fold) in response to an egg white environment. In this study, the potential role of AcrD in the resistance of S. Enteritidis to egg white was explored by gene deletion, survival ability test, morphological observation, Caco-2 cell adhesion and invasion. It was found that deletion of acrD had no apparent effect on the growth of S. Enteritidis in Luria-Bertani (LB) broth but resulted in a significant (p < 0.05) decrease in resistance of S. Enteritidis to egg white and a small number of cell lysis. Compared to the wild type, a 2-log population reduction was noticed in the ΔacrD mutant with different initial concentrations after incubation with egg white for 3 days. Furthermore, no significant difference (p > 0.05) in the adhesion and invasion was found between the wild type and ΔacrD mutant in LB broth and egg white, but the invasion ability of the ΔacrD mutant in egg white was significantly (p < 0.05) lower than that in LB broth. This indicates that acrD is involved in virulence in Salmonella. Taken together, these results reveal the importance of AcrD on the resistance of S. Enteritidis to egg white.
Highlights
Eggs are an important and integral part of the human diet
To test whether acrD was up-regulated in the whole egg white (i.e., 100% egg white), the expression of acrD in S
These results demonstrate that acrD confers resistance to egg white in S
Summary
Eggs are an important and integral part of the human diet. These are consumed all over the world and possess natural physical and chemical defenses to prevent the contamination of microorganisms [1]. Previous works have demonstrated that Salmonella usually infects the human host through the ingestion of contaminated food products This bacterium is able to resist the adverse environment of the gastrointestinal tract and adhere, colonize and invade host intestinal epithelial cells, leading to human infections and diseases [19,20]. S. Enteritidis to egg white by gene expression analysis, gene deletion, survival ability test, cellular morphology analysis, Caco-2 cell adhesion and invasion assays. Enteritidis to egg white by gene expression analysis, gene deletion, survival ability test, cellular morphology analysis, Caco-2 cell adhesion and invasion assays These results will provide new information to help elucidate the resistance mechanisms of S.
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