Research on Mechanism of Curcumin with Chitosan Nanoparticles in Regulating the Activity of Podocytes in Diabetic Nephropathy Through Alleviating Oxidative Stress and Inflammation

Abstract

The aim of this study was to assess the mechanism of curcumin with chitosan nanoparticles in regulating the activity of podocytes in diabetic nephropathy through alleviating oxidative stress and inflammation. MCP-5 cells were cultivated in vitro after being randomly divided into four sets, including control set, high sugar set, curcumin set and nanometer set. Proliferation was detected and apoptotic condition was detected through testing activity of Caspase 3. The activities of malondialdehyde (MDA) and superoxide dismutase (SOD) were also detected, and also the ROS content. Expressions of TNF-α, IL-6 and IL-10 were detected through enzymelinked immunosorbent assay (ELISA) method, and mRNA and protein expression of podocin was detected. Results showed that the proliferation of podocytes was prompted in the curcumin set and set of curcumin with chitosan nanoparticles, while the activity of Caspase 3 was reduced. Moreover, the contents of MDA and ROS were reduced, while the SOD activity was increased. The presentation of TGF-β1, and secretions of TNF-α and IL-6 were reduced, while the secretion of IL-10 was increased and presentation of podocin was increased. The activity of podocytes in diabetic nephropathy was improved by curcumin with chitosan nanoparticles through alleviating the oxidative stress and inflammation. The apoptosis was reduced. The development of diabetic nephropathy could be therefore effectively improved.