Report of forensically relevant insects collected from pig and rabbit remains during two forensic entomology workshops in Eastern Massachusetts.

Abstract

Forensic entomology relies on known geographic ranges and seasonal presence of forensically relevant insects. In the Northeastern United States, there is no information on species in the region in early spring. Two forensic entomology workshops took place in April of 2023 and 2024 in Milton, Massachusetts. During this workshop, practitioners were trained in the appropriate collection and storage techniques prior to a practical experience including the collection of insects from pig and rabbit remains. All insects collected were identified down to family or species level. Across both years, Phormia regina (Meigen) (Diptera: Calliphoridae) was the fly consistently colonizing remains and was the oldest immature larvae in all instances. Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae), Protophormia terraenovae (Robineau-Desvoidy) (Diptera: Calliphoridae), and Muscina stabulans (Fallén) (Diptera: Muscidae) were additional flies co-colonizing remains with P. regina. Other species were found as adults surrounding the remains that included other necrophagous fly species, beetles, and ants. Variation in insects collected between years likely due to high temperature variation. This documentation of species provides context to forensically related flies expected in Eastern Massachusetts and provides research directives in the region.