Abstract
Parental RNA of the bacteriophage R17 is converted to a double-stranded replicative intermediate which can be detected by its characteristic sedimentation rate and its resistance to pancreatic ribonuclease as early as six minutes after infection of Escherichia coli. Chloramphenicol inhibits the conversion and therefore production of the ribonuclease-resistant form must require protein synthesis. Prior to ribonuclease treatment the replicative intermediate displays a heterogeneous sedimentation characteristic in sucrose gradients, but it sediments as a homogeneous sharp peak if treated with RNase before centrifugation. Sedimentation analysis of lysates of infected cells prepared 20 minutes after infection demonstrates that all intact parental phage RNA found in the lysate sediments with 70 s ribosomes in both the single- and double-stranded forms.
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