Abstract
Melipona bees are remarkable due to the high contrast in heterochromatin amounts, making this group interesting for studying repetitive DNA amplification. Here, we performed the first efforts for the chromosomal localization of different repetitive DNAs in M. (Michmelia) scutellaris and tested for unique or multiple heterochromatin amplification in Melipona subgenera. Our data revealed enrichment of repetitive DNAs in chromosomal heterochromatic arms demonstrated by C0t-DNA and DOP-PCR probe hybridization, although microsatellites and multigene families were located at terminal euchromatic regions. Analysis using C0t-DNA probe from M. scutellaris showed positive hybridization only in Michmelia species, suggesting monophyletic amplification and sharing of heterochromatin sequences between species. However, the subgenus Melikerria, with a high amount of heterochromatin, probably underwent independent heterochromatin amplification or experienced sequence modification.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
