Remnant Epitope Autoimmunity in Human Abdominal Aortic Aneurysm: A Pilot Study with Elastin Peptides

Abstract

Abdominal aortic aneurysm (AAA) is a prevalent disease affecting around 5% of the population aged more than 65years. The exact etiology and physiopathology of AAA still raises questions, and elective surgery is currently the only treatment option for this often progressive disease. In this study, we hypothesized and tested a pathophysiological model that depicts AAA as an inflammation-triggered autoimmune disease with remnant vessel wall peptide fragments as the antigen. A pilot study with male AAA patients (n=14) and male controls (n=8) was conducted. In both study groups, peripheral blood monocytes and plasma were separated from whole blood by centrifugation. An ELISpot test was performed on cultured white blood cells for the presence of elastin-specific T-lymphocytes. An Enzyme-linked immuno sorbent assay (ELISA) was performed on plasma for the presence of elastin-specific IgG molecules. ELISpot interferon-gamma secretion in AAA (7.7±9.5%) and control (4.6±3.5%) and ELISA anti-elastin IgG titer in AAA (77.5±17.8%) and control (78.2±31.5%) were not significantly different (P=0.94 and P=0.55, respectively). Both results are expressed as a percentage relative to the respective positive and negative control. The results of our pilot study did not indicate a clear and invariable autoimmune process directed against remnant elastin peptide fragments. Further research into the model mechanics and a possible antigen is still necessary. In the mean time, the model as presented here already offers a pathophysiological framework to further research into the possible remnant epitope-driven AAA etiology.