Abstract
To study the Ca + dependency of luteinizing hormone-releasing hormone (LHRH) release in the bullfrog sympathetic ganglia, a method was developed to fill the preganglionic nerve terminal boutons with membrane-impermeant fura-2. We found that as stimulation frequency increased from 0.5 to 40 Hz, the peak [Ca 2+] i ([Ca 2+] p) and the rate of rise in [Ca 2+] i increased, the decay of [Ca 2+] i transients followed up to three exponentials, and release of LHRH was linearly related to ƒ([Ca 2+] i - [Ca 2+] t)dt. The threshold level of [Ca 2+] i for LHRH release for a given set of boutons on a C cell, [Ca 2+] t, was estimated by the [Ca 2+] p evoked by 0.5 Hz stimulation that does not induce LHRH release.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
