Abstract
WHEN the distribution of active materials in vertebrate tissue is being studied, it is necessary to free tissue extracts from substances which interfere with the bioassay of 5-hydroxytryptamine. Garven1 has described a method for the destruction of polyphenols by the polyphenol oxidase present in mushroom juice. The juice contains both polyphenol and monophenol oxidase, and her method therefore makes use of the faster inactivation of adrenaline and noradrenaline by polyphenol oxidase than of 5-hydroxytryptamine by monophenol oxidase. The present method depends on the differential stability of 5-hydroxytryptamine, adrenaline and noradrenaline in alkaline solution.
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