Relationship between host Mycobacterium tuberculosis-specific CD4 T cell responses and sputum bacillary clearance during early anti-tuberculosis treatment

Abstract

Event Abstract Back to Event Relationship between host Mycobacterium tuberculosis-specific CD4 T cell responses and sputum bacillary clearance during early anti-tuberculosis treatment David T. Mzinza1*, Derek Sloan1, 2, Katalin Wilkinson3, Robert Wilkinson3, Gerry Davies1, 2, Robert Heyderman1 and Henry Mwandumba1, 2 1 Malawi Liverpool Wellcome Trust Clinical Research Programme, Malawi 2 University of Liverpool, United Kingdom 3 University of Cape Town, South Africa Tuberculosis treatment is associated with changes in host Mycobacterium tuberculosis (Mtb) antigen-specific T cell responses. It is unclear how these changes relate to treatment outcomes. We investigated the relationship between Mtb-specific immune response, quantitative bacteriology and clinical outcomes of treatment in sputum smear-positive adult TB patients receiving anti-TB treatment at Queen Elizabeth Central Hospital, Malawi. Peripheral blood and sputum samples for PBMC isolation and serial mycobacterial culture respectively were collected from 45 patients irrespective of their HIV status before and after 56 days of treatment. Mtb-specific immune responses were assessed by quantification of IFN-γ secreting T cells in response to CFP-10 and ESAT-6 using an ELISPOT assay. Mtb clearance from sputum was evaluated by mycobacteria growth indicator tube culture system. Results are given as median and differences were considered statistically significant if P≤0.05. Preliminary results show a difference in the rate of bacillary clearance and host responses with respect to HIV. 71% of HIV-uninfected and 50% of HIV-infected patients were culture negative by 2 months of treatment. Similarly, the number of IFN- secreting T cells reduced during treatment in HIV-uninfected patients (median change -60 SFU/106 PBMC, IQR 4-204), while there was a smaller reduction in HIV-infected patients (median change -14 SFU/106 PBMC, IQR 0-51) (Mann-Whitney U-test p = 0.085). Preliminary findings suggest an association between magnitude of host Mtb-specific T cell responses and mycobacterial load. We plan to analyze the data further by doing regression modeling to investigate the relationship between immunological parameters, HIV status and bacillary clearance. Keywords: Tuberculosis, T cell, IFN- γ, CFP-10, ESAT-6 Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Mzinza DT, Sloan D, Wilkinson K, Wilkinson R, Davies G, Heyderman R and Mwandumba H (2013). Relationship between host Mycobacterium tuberculosis-specific CD4 T cell responses and sputum bacillary clearance during early anti-tuberculosis treatment. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00095 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 09 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mr. David T Mzinza, Malawi Liverpool Wellcome Trust Clinical Research Programme, Blantyre, Malawi, dmzinza@mlw.mw Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers David T Mzinza Derek Sloan Katalin Wilkinson Robert Wilkinson Gerry Davies Robert Heyderman Henry Mwandumba Google David T Mzinza Derek Sloan Katalin Wilkinson Robert Wilkinson Gerry Davies Robert Heyderman Henry Mwandumba Google Scholar David T Mzinza Derek Sloan Katalin Wilkinson Robert Wilkinson Gerry Davies Robert Heyderman Henry Mwandumba PubMed David T Mzinza Derek Sloan Katalin Wilkinson Robert Wilkinson Gerry Davies Robert Heyderman Henry Mwandumba Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.